Japanese researchers have recently developed an artificial nucleic acid that can identify nucleic acids with specific base sequences. With the help of such artificial nucleic acid, the researchers observed the activity of RNA (ribonucleic acid) in living cells.
To explore the function of cells, humans have developed a wide variety of fluorescent proteins so far.
If you want to observe a cell, fluorescent protein can combine with the molecules inside this cell, and then emit a fluorescent signal, so that people can clearly observe the activities of various substances in living cells. However, since nucleic acids such as RNA in cells are too small, existing fluorescent proteins are not suitable for "staining" them.
According to a Japanese media report on the 23rd, in order to solve the problem of clearly observing the RNA of living cells, researchers at the Japan Institute for Basic Research thought of using the principle of "exciton interaction" that can lead to the transformation of the charge properties within molecules. They developed an artificial nucleic acid based on organic fluorescent molecules with the function of "exciton interaction". This artificial nucleic acid can recognize the nucleic acid with a specific base sequence and combine with the target nucleic acid to emit fluorescence through "exciton interaction".
According to reports, for different base sequences, researchers have also developed artificial nucleic acids that can emit different colors of fluorescence, and successfully used this fluorescence system to observe the activity of RNA in human cervical cancer tissue cells in real time.
The relevant papers of the above research were published in the online version of the German "Applied Chemistry" on the 23rd.
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Beijing Jiehui Biotechnology Co., Ltd. is established on the basis of the State Key Laboratory. It is a high-tech enterprise co-founded by senior personnel with backgrounds in life sciences, biotechnology, and returned overseas students.
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