Guyan Reagent-Yizhi Biochemical Reagent

Puzzle [Pinyin Name] Yì Zhì [English Name] FRUCTUS ALPINIAE OXYPHYLLAE [Alias] Yizhiren, Yizhizi [Source] This product is a dried and ripe fruit of the Alpinia oxyphylla Miq. In summer and autumn, the fruit is harvested when it changes from green to red, dried in the sun or dried at low temperature. 【Properties】 This product is oval, slightly pointed at both ends, 1.2 to 2 cm long, and 1 to 1.3 cm in diameter. The surface is brown or gray-brown, with 13 to 20 longitudinally protruding ridges, perianth residues at the top, and fruit pedicels often remaining at the base. The pericarp is thin and slightly tough, close to the seeds, and the seeds are gathered into a cluster. There are diaphragms to divide the seed cluster into 3 petals. Each petal has 6 to 11 seeds. The seeds are irregularly oblate, slightly obtuse, about 3mm in diameter, and have a grayish brown or grayish yellow surface. They are covered with a light brown membranous aril; they are hard and endosperm white. It has a peculiar aroma, is pungent, and slightly bitter. [Identification] (1) Cross section of the seed: Parenchyma cells of the aril sometimes remain. Seed coat epidermal cells are round, square or rectangular, slightly radially extended, and thicker walls; the hypodermis is 1 row of parenchyma cells, containing yellowish brown; 1 row of oil cells, square or rectangular, containing yellow oil droplets ; The pigment layer is a series of yellow-brown cells, with 1 to 3 columns of large round oil-like cells scattered between them, containing yellow oil droplets; the inner seed coat is a row of palisade thick-walled cells, yellow-brown or red-brown, the inner wall and The side walls are extremely thick, the cells are small, and they contain siliceous blocks. The endosperm cells are filled with starch clusters formed by tiny starch granules. Endosperm cells contain slurries and fatty oil droplets. The powder is yellowish brown. The surface of the epidermal cells of the seed coat is elongated, with a diameter of about 29 μm, and a slightly thicker wall, often arranged vertically with the upper and lower layers of the hypodermal cells. Pigment layer cells shrink, the boundaries are unclear, and contain reddish brown or dark brown objects, often broken into irregular pigment blocks. Oil cells are square, rectangular, or scattered between cells in the pigment layer. Endothelial thick-walled cells are yellow-brown or brown, polygonal in surface view, thick in wall, non-woody, and contain siliceous blocks in the cell cavity; one row of cells in the cross-sectional view, grid-like, with extremely thick inner wall and side wall, and partial cell cavity The outside contains siliceous blocks. The endosperm cells are filled with starch clusters formed by tiny starch granules. Endosperm cells contain slurries and fatty oil droplets. (2) Take the volatile oil under [Content Determination] and add absolute ethanol to make a solution containing 10μl per 1ml as the test solution. Take another puzzle control medicinal material and make a control medicinal solution in the same way. According to the thin layer chromatography (Appendix Ⅵ B) test, draw 5 to 10 μl of each of the above two solutions, and spot them on the same silica gel GF254 thin layer plate, using cyclohexane-ethyl acetate (9: 1) as the developing agent. Unfold, remove, dry, and inspect under UV light (254nm). In the chromatogram of the test product, the same dark spots were shown at the corresponding positions of the chromatogram of the control medicinal material; sprayed with dinitrophenylhydrazine ethanol test solution and left for a while, the spots gradually turned orange-red. [Determination of content] Take this seed and determine according to the determination method of volatile oil (Appendix XX D). The volatile oil of this variety shall not be less than 1.0% (ml / g). 【Processing】 Yi Zhiren: Remove impurities and shells. Mash up in time. Salt Yizhiren: Take Yizhiren, stir-fry it according to the salt water roasting method (Appendix II D), and mash it in time. [Sexual flavor] Xin, warm. 【Return to the Channel】 Return to the spleen and kidney. [Functions] Warm the spleen and stop diarrhea, take saliva, warm the kidney, solidify the urine. It is used for diarrhea due to cold spleen, cold pain in the abdomen, salivation in the mouth, kidney enuresis, frequent urination, and spermatorrhea. 【Usage and dosage】 3 ~ 9g. [Storage] Store in a cool and dry place.

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