The Elisa test is an experimental diagnostic method with high sensitivity, strong specificity, and good repeatability of the elisa kit. Due to its stability, easy storage, easy operation, and objective judgment of results, it has been widely used in various fields of immunological testing. The ELISA detection kit is used for qualitative detection of anti-human hepatitis E (HEV) virus IgM antibody in human serum or plasma in vitro by ELISA. elisa kit Our company sells a variety of elisa kits, including rat, mouse, human, guinea pig, chicken, and pig. There are all kinds of elisa kits. The company's unified specifications are 48T, 96T, and human ELISA kits. The steps are not the same, the following are the specific steps to share:
ELISA kit operation steps (general):
1. Add standard products: set up standard wells on the enzyme-coated plate. Elisa kit ELISA kits are successively added with 50ul of standard samples of different concentrations (recommended to make 2 parallel wells for each concentration) human ELISA kits.
2. Adding samples: set up blank wells (the blank control wells do not add samples and enzyme reagents, the rest of the steps are the same) and the sample wells to be tested. In the rat ELISA kit, add 40 μl of the sample to the test sample well of the enzyme-coated plate, and then add 10 μl of the sample avidin. Add the sample and add the sample to the bottom of the well of the microplate. Try not to touch the wall of the well. The elisa kit is gently shaken to mix the human ELISA kit.
3. Color development: Add 50μl of developer A to each well, and then add 50μl of developer B, mix gently, and develop the color for 15 minutes at 37 ° C in the dark and avoid human ELISA kit.
4. Termination: Add 50μl of stop solution to each well to stop the reaction (at this time, the blue color turns to yellow).
5. Incubation: Seal the plate with a sealing plate and incubate the ELISA kit at 37 ° C for 30 minutes.
6. Mixing solution: Dilute 30 times concentrated washing liquid with distilled water 30 times and reserve.
7. Washing: Carefully peel off the sealing film, discard the liquid, and spin dry. Each well of the Elisa kit rat ELISA kit is filled with the washing solution, let it stand for 30 seconds and discard it. Repeat this 5 times and pat the mouse dry ELISA kit.
8. Add enzyme: add 50μl of enzyme label reagent to each well, except the blank well of elisa kit, except the ELISA kit.
9. Incubation: The operation is the same as 3.
10. Washing: The operation is the same as 5.
Measurement: The absorbance (OD value) of each well was measured in sequence with a blank air conditioner at zero and a wavelength of 450 nm. The measurement should be carried out within 15 minutes after adding the stop solution.
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