Detection range: 96T0.6U / mL-20 U / mL Purpose: This kit is used to determine the content of promelanocortin (POMC) in goldfish serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of protomelanin (POMC) in the specimen. The microplate was coated with purified goldfish amelanin (POMC) antibody to make a solid-phase antibody, and then the amelanin (POMC) was added to the monoclonal antibody-coated microwells in turn, followed by the Promelanin (POMC) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The shade of the color is positively correlated with the promelanin (POMC) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of protogenin (POMC) in the sample was calculated by a standard curve. Kit composition 1 30-fold concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 enzyme label reagent 6ml × 1 bottle 8 standard (40 U / mL) 0.5ml × 1 bottle 3 enzyme label coated plate 12 wells × 8 strips 9 standard diluent 1.5ml × 1 bottle 4 sample diluent 6ml × 1 bottle 10 instruction manual 1 copy 5 developer A solution 6ml × 1 bottle 11 sealing film 2 sheets 6 developer B solution 6ml × 1 / Bottle 12 sealed bags 1
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