Suzhou Medical Institute developed a new sensor for detecting "clenbuterol"

Clenbuterol, commonly known as "clenbuterol", is a potent and selective beta-agonist that promotes the synthesis of proteins in skeletal muscle, strengthens the breakdown of body fat and inhibits its synthesis. After the animal is eaten, it can effectively reduce the deposition of fat in the body and increase the ratio of lean meat to fat in the ketone body. Therefore, some unscrupulous traders use it in animal husbandry, resulting in the accumulation of Clenbuterol in animals, especially in liver tissue. After eating this kind of meat products, Clenbuter can break down the fat into free fatty acids into the blood, reduce the elasticity of the blood vessel wall, cause the blood pressure to rise, cause the microcirculation blood vessels to swell, compress the nerves, and express muscle vibration, palpitation, Trembling, headache, nausea, vomiting and other symptoms. Based on its harm to human health, the use of Clenbuterol in animal husbandry is strictly prohibited in China. However, the current illegal use situation is very serious, so it is especially important to develop sensitive and convenient detection methods.

At present, the commonly used clenbuterol detection methods include high performance liquid chromatography, gas chromatography-mass spectrometry, enzyme-labeled immunosorbent assay, capillary electrophoresis, and solid phase extraction. However, the large equipment required for the above method is relatively expensive, complicated to operate, and time consuming and labor intensive.

Recently, the Wang Yushen Research Group of the Suzhou Institute of Biomedical Engineering and Technology of the Chinese Academy of Sciences has developed a new type of electrochemical sensor for the specific detection of clenbuterol. They modified 1.3.5-triazine-2.4.6-triamino on the surface of the synthesized silver nanoparticles and the surface of the gold electrode, which formed multiple hydrogen bonds with the clenbuterol molecule. Thus functionalized silver nanoparticles can be used as specific recognition molecules for clenbuterol. When the Clenbuterol is present in the test sample, the functionalized silver nanoparticles will be immobilized on the gold electrode surface and provide a good electrochemical signal. The sensor has a detection limit of 10 pM and a linear detection range of 10 pM to 100 nM and can be used for Clenbuterol detection in biological samples with various interfering substances.

Relevant research results have been published online in the journal ACS Applied Materials & Interfaces.

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