The classic method of ensuring Tibetan microbial strains is downloaded

Subculture and preservation method Some microorganisms die quickly when they are subjected to treatment such as freezing or drying. Therefore, in this case, only the subculture preservation method can be resorted to. Subculture is to regularly carry out the transfer of strains, and then preserve them after cultivation. It is the most basic method of microbial preservation, such as the preservation of commonly used strains such as yogurt. When subcultured, the concentration of the medium should not be too high, and the nutrient content should not be too rich, especially the concentration of carbohydrate should be reduced as much as possible. The culture temperature is usually preferably slightly lower than the optimum growth temperature. If it is an acid-producing strain, a small amount of calcium carbonate should be added to the medium. Generally, the preservation temperature of most strains is preferably 5 ° C. Microorganisms such as anaerobic bacteria, Vibrio cholerae and some pathogenic fungi can be stored at 37 ° C, while strains of large edible fungi such as mites It can be stored directly at room temperature. ·The subculture preservation method is simple, but its shortcomings are also obvious: 1. The fungus tube tampon is often prone to mildew; 2. The genetic traits of the strain are prone to variability; 3. The pathogenicity and physiological activity of the strain are repeated. The ability of the substance and the ability to form spores are reduced; 4. It is necessary to switch regularly and the workload is large; 5. There are many opportunities for contamination of the bacteria. There are also a variety of save method content, because the number of words is limited, to understand more and more ways to save the method, you are welcome to download this article, click on the download to save the file for free! Remember to unzip the file after downloading! Shanghai Hengyuan welcomes you!

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