Improvement of cell cryopreservation method

Cell cryopreservation is one of the important links in cell culture. Using cryopreservation technology to store cells at -196 ° C in liquid nitrogen for cryopreservation can temporarily remove the cells from growth and preserve their cell characteristics, so that they can be used for experiments when needed. Moreover, a certain amount of cells can be kept in moderation, which can prevent the cells being cultured from being contaminated or other unexpected events that can cause the cells to lose seeds and play a role in cell preservation. The main point of the cryopreservation technique is slow freezing. The standard freezing procedure is a cooling rate of -1 ~ -2 ℃ / min; when the temperature is below -25 ℃, it can be increased to -5 ℃ ~ -10 ℃ / min. The traditional method we commonly use is to place the cryotube at 4 ℃ for 30 minutes ---> -20 ℃ for 60 minutes ---> -80 ℃ overnight ---> liquid nitrogen tank. But because of the many steps and the long time interval, it is easy to forget. But the delay of any one step will seriously affect cell survival. The procedure freezer on the market is expensive and not necessary, not every laboratory has it. Method improvement: We take into account that the foam plastic has a good thermal insulation effect, and at the same time noticed that the pore size of the foam plastic in the 15ml centrifuge tube package of the BD company we purchased was equivalent to that of the cryopreservation tube. Our improved cryopreservation method is: put the cell cryopreservation tube into a foam holder, and then add a piece of the same foam holder, put it directly into the -80 ℃ refrigerator overnight and transfer it into a liquid nitrogen tank. This method not only utilizes waste for environmental protection, but also saves time and hassle, and is very convenient and effective.

We are professional manufacturer, which is established in 2005 and focus on export business to Euope, USA and Australia.

We specialized in producing various kinds of tissue products, including Family Box Tissue, Cube Box Tissue, Cylider car tissue, Mansize Box Tissue, Soft Pack tissue and Flat Pack Tissue etc. 

We provide customized service. We can make box at any size and shape as well as tissue size.

We supply different kinds of materials for soft pack tissue, like Virgin wood pulp,  Recycled Pulp, Bamboo Pulp (Tree free).

The Material that we used are 100% biodegradable and compostable, Chlorine free and reach European food contact grade standard.

Soft Pack

Soft Paper,Soft Pack,Soft Tissue Paper,Ultra Soft Toilet Paper

Bobo Tissue Product Manufacturer , https://www.bobotissues.com

Posted on